IAEA-CN-115-35 · Electron Beam Sterilization of the Agarose Gel Used for Electrophoresis

D. Ighigeanu¹, D. Martin¹, E. Manaila¹, D.E. Stan², I. V. Baciu³, G. Craciun¹, C. Oproiu¹, N. Iacob¹

¹National Institute for Laser, Plasma and Radiation Physics, Electron Accelerators
Laboratory, #409 Atomistilor St., P.O.Box-MG36, 077125 Magurele, jud. Ilfov, Romania
²Clinical Hospital „Caritas” , #29 Traian St., sector 3, Bucharest, Romania
³University of Bucharest, Chemistry Faculty, #90-92 Panduri St., sector 5, Bucharest,
Romania

Abstract: The results obtained by electron beam (EB) sterilization of the plates with agarose gel used for human serum protein electrophoresis are presented. Also, the results obtained by human serum protein electrophoresis performed with agarose gel plates irradiated at different EB doses, from 4 kGy to 20 kGy, are presented. The microbiological results demonstrate that above 5 kGy the irradiated agarose plates are sterile. The EB irradiation of the agarose gel plates in the dose range of 7-9 kGy gives the best results for both, sterilization and protein fraction separation processes.

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