This publication draws on the results of a coordinated research project on comparative analysis of methods and tools for knowledge preservation in nuclear organizations. The project was initiated by the IAEA in order to enhance the capacity of Member States to maintain and preserve the information and knowledge resources related to the peaceful uses of nuclear energy. The project participants explored methods and tools used to capture, interpret, analyse and disseminate data and information, as well as the knowledge ultimately derived from them. Furthermore, a survey tool on the current status of knowledge preservation in nuclear and supporting organizations was developed. The analysis of the survey served as a basis for the recommendations and conclusions on good practices in knowledge preservation.