The guidebook gives a complete survey of non-destructive techniques available for application in the spent fuel pool as well as in hot cells, thereby supplementing an earlier publication, IAEA- TECDOC-245. It provides information on methods of examining water reactor fuel and a basis for further innovations. Contents: 1. Introduction; 2. Defect surveillance and detection; 3. Visual inspection; 4. Dimensional measurements; 5. Oxide layer thickness and crud measurements; 6. Gamma scanning; 7. Neutron radiography and X ray radiography; 8. Fission gas determination; 9. Transfer, collection, documentation and storage of post-irradiation data; 10. Conclusions.