This publication contains information on the dosimetry and monitoring of tritium, the use of protective clothing for work with tritium, safe practices in tritium handling laboratories and details of tritium compatible materials. The information has been compiled from experience in the various applications of tritium and should represent valuable source material to all users of tritium, including those involved in fusion R&D. Contents: 1. Introduction; 2. Radiological hazards and dosimetry; 3. Tritium monitoring; 4. Personal protective clothing; 5. Properties and compatibility of materials for safe practice in tritium containing systems; 6. Management of tritiated wastes; 7. Safe handling of tritium in laboratories; Annex I: Gaseous tritium light source manufacturing; Annex II: Commercial radiochemical manufacturing; Annex III: Safe handling of tritium in low and medium level university and medical facilities; Annex IV: Tritium safe handling in heavy water reactors.